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Peptides were added in 10-fold molar excess compared to the 4A6 antibody, in a final volume of 380 µl of 1% CHAPS, 1% NP-40.
THL concentration was increased to a maximum of 25 µM (a molar excess compared to enzyme of 505) in an attempt to detect inhibition.
Unlabeled probes were used at a 200-fold molar excess compared to labelled probe.
GKRP is present in liver cells in a 2 3-fold molar excess compared with GK, and its expression is relatively independent of food intake and hormonal status.
An 8-fold higher (w/w) concentration of MAC (177.8 μM, 74-fold molar excess) compared to that of HGD showed dramatic suppression of aggregation.
In the latter profilin was present in a 15-fold molar excess compared to actin [ 48] and this also appears to be the case in lupin.
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At 50- to 100-fold molar excesses compared to proMMP-2, P33-42 diminishedy diminished the activating effect of (GPO)10 on proMMP-2.
The results indicate that the mRNA is present in ∼30-fold molar excess as compared to the ncRNA.
In this test, soluble scFv was used at concentrations up to 250 μg ml−1 (giving an A490 nm ranging from 1 to 1.5 by ELISA), that is, 10 μ M, corresponding to a 1000-fold molar excess as compared with the soluble HER-2/neu ECD-Fc fusion antigen.
Labelling was optimized with highest yields at a 10-fold molar excess of DTPAA compared to the proteins and a 2-fold molar excess of Ce and Sm compared to DTPAA-protein.
Theoretical calculations estimating the percentage of protein-bound peptide from its IC50 value revealed that a 130-fold molar excess of peptide compared to the protein had to be used to ensure sufficient occupancy of the protein with peptide.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com