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Spectrophotometric methods rely on the molar absorptivity (or the molar absorption coefficient) of pure nucleic acid solutions used in the equation based on the Lambert-Beer law [ 9].
SOA exhibited a low molar absorptivity of 439 absorption units/cm/M in water at 210 nm requiring low-wavelength UV-DAD detection.
The shape of absorption bands is similar, but the molar absorptivity in case of TSC substituent is almost 1.5 times higher compare to MTSC.
K2FeO4 dissolved as FeO42− has the absorption peak at 510 nm, and its molar absorptivity at 510 nm has been determined as 1150 M−1 cm−1 [ 14].
In its monomeric state, AmB exhibits a characteristic absorption at 409 nm with an apparent molar absorptivity that decreases upon aggregation.
Wavelengths of maximal absorption for each drug in different media plus drug molar absorptivity were determined from analysis of optical spectra.
The bending mode is particularly affected at lower signal levels because of its weaker molar absorptivity, and the fact that the frequency range overlaps with water vapor absorption, making it more susceptible to errors in the thin sheet limit.
Protein concentrations were determined from the absorbance at 280 nm using a molar absorptivity constant of each protein.
where \(\varepsilon\) is the molar absorptivity, c is the concentration of water (55.5 M), and l is the sheet thickness.
Protein concentrations were determined from the absorbance at 280 nm using a molar absorptivity constant (ε280 = 103,280 M−103,280.
If we use the maximum absorbance value with the literature molar absorptivity at 3404 cm−1, the calculated sheet thickness is increased only slightly to 17 ± 2 nm.
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