Sentence examples for molar DNA from inspiring English sources

Finally, the PCR products from each treatment were mixed to obtain equal molar DNA for sequencing.

For assessment of neurite length, cultures were co-transfected at DIV 3 with V5-tagged human VPS35 and GFP plasmids at a 10 1 DNA molar ratio (5 µg total DNA per 35 mm dish) using Lipofectamine 2000 reagent (Invitrogen).

It was observed that interaction of HupBMtb with supercoiled pBSK at a molar ratio (protein: DNA) of 75∶1 the intensity of DNA protein complex was large and the DNA remained lodged in the well (lane 4).

At a 2 1 protein:DNA molar ratio, the c(s) distribution showed a predominant species at 5.276 S. At a 3 1 protein:DNA molar ratio, the additional slower peak is at 2.33 S, corresponding to free PhoP.

Molar concentrations of DNA are given in terms of total nucleotides.

Even at the highest protein to DNA molar ratio sufficient amount of unbound pBSK plasmid was visible (Figure 4A, lane 7).

The optimal VP1 DNA ratio on a weight basis, 5∶1, corresponded to a 1∶1 capsid to DNA molar ratio (MW of a VP1 capsid is ∼15 Mda, and of a 5 Kbp DNA molecule is ∼3 Mda).

A comparison of these calculations and molar intensities of DNA bands in experiment has shown a similarity of both ratios.

Neurons were transfected at DIV 3 with combinations of FLAG-LRRK2, Myc-Drp1/Myc-Mfn1/Dnm1-GFP anDsRed-Maxax plasmids at a 10 10 1 DNA molar ratio (5 µg DNA total/dish) using Lipofectamine 2000 reagent (Invitrogen).

Enhancement of gene transfer efficiency depended on IBB peptide / plasmid DNA molar ratio: reporter gene expression increased with the amount of added IBB peptide.

Here we report a simple, high-throughput, and cost-effective sequencing method that includes normalized library preparation and adjustment of DNA molar concentration.