Serum (4 mL) was diluted with 4 mL equilibrium buffer of 0.02 mol/L ammonium acetate (pH 6.8), which was applied to a heparin-Sepharose column (1.0 × 10 cm; Pharmacia, Uppsala, Sweden).
A portion of standard sample, water sample, or digested herbal sample solutions containing the studied ions were added into a 50-mL beaker, and the pH of the mixture was adjusted to the desired value with 0.1 mol L−1 HCl and/or 0.1 mol L−1 NH3.H2O, and the mixture was diluted to 10 mL with deionized water.
The supernatant was diluted 10 times with 0.1 mol L−1 Tris-HCl/2 mmol L−1 EDTA solution buffer to lower the ethanol concentration.
The hydrolyzed urine sample was diluted with 1 mL phosphate buffer (0.1 mol L−1, pH 2.4) [ 7].
After incubation for 30 minutes at room temperature in the dark, the mixture was diluted with 50 mmol l−1 ammonium bicarbonate buffer at a final concentration of 1 mol l−1 urea.
The appropriate concentrations of HSA, NaCl, and patulin 15 μmol ·L−1, 1.0 mol ·L−1, and 1.0 mmol ·L−1, respectively were added to a 10 mL volumetric flask; next, the mixture was diluted to graduation with Tris-HCl buffer solution.
Big East basketball was diluted.
Every QD colloidal solution was diluted to a constant concentration of nanoparticles (2 × 10−6 mol/l).
Stock solutions of estrone, estradiol, and estriol were firstly dissolved using several drops of 0.01 mol/L NaOH solution and the working standard solution was diluted with water.
Before determination, the sample was diluted with 0.01 mol/l phosphate buffer pH 7.0 (Ransod, Randox, UK).
