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Alkyne-functionalized pyrene with a photocleavable 2-nitrobenzyl moiety was added to the PEO-b-PAzHEMA backbone via click chemistry to produce the desired block copolymer with high fidelity.

A single 39 adenosine moiety was added to the cDNA using Klenow exo and dATP.

Strikingly, when the R9 moiety was added to the fusion protein it enhanced cell killing 10-fold over that attained with CPE-G4S-rGel alone (IC50 78 nM versus 715 nM).

Then, a synthetic substrate for a-CT (N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide) carrying a chromogenic moiety, was added and the optical density at 405 nm was determined (Beckman DU640 spectrophotometer, Fullerton, CA).

Similar(56)

Initially a farnesyl moiety is added to the cysteine by farnesyltransferase, followed by cleavage of the last three amino acids (-aaXing) by either Rce1 or ZmpSte24.

To produce the biotinylated tetravalent antigen b-EDIII-T, we used an in vivo method wherein the biotinyl moiety is added to a specific site enzymatically [ 8].

Regulation of these palmitoylation targets could involve cyclic acylation, like H/NRas (Rocks et al., 2005), or they could be targets of "palmitoyl switches," with a palmitoyl moiety being added or revealed after phosphorylation or ligand biding (Salaun et al., 2010).

We note that the rate constants measured in the in vitro assay reflect all of the processes that can occur each time a new lysine moiety is added to the growing polypeptide chain (Lys-tRNALys binding, peptidyl-transfer, translocation, peptidyl-tRNA drop-off, 70S complex instability, etc).

Sequences encoding a ZZ [a tandem IgG binding domain based on S. aureus protein A (Nilsson et al, 1987)] and SNAPf moiety were added to the 5′ end of the DHC gene, producing tags on the dynein tail that permit affinity purification and covalent labelling with bright fluorophores, respectively.

Since no structural model of quinupristin was available, virginiamycin S was used as an initial model; the quinuclidinylthio-moiety was added manually and subsequently minimized.

A solution of GFP-CVIA, GST-CVIA, or proG-CVIA modified at the C-terminal cysteine with farnesyl (GFP-CVIAf and GST-CVIAf) or propargylfarnesyl (GFP-CVIApf, GST-CVIApf, and proG-CVIApf) moieties was added to individual wells, followed by 0.5 mM CuSO4, 50 μM TBTA, and 0.5 mM TCEP (final volume 10 μL).

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