The binary toxin produced by B. sphaericus may be synthesized as 110- 125-KDa protein which is converted to a non-toxic 63-KDa moiety and a toxic 43-KDa moiety during the process of sporulation.
Our results are consistent with structural rearrangements in which there is an 8.5-Å closure between the 344W residue and the mant moiety during the transition from the strongly (ADP) to weakly (ATP) actin-bound states of the myosin ATPase cycle.
The dimers are only connected via hydrogen bonds at the C-terminal moiety during the whole simulation.
However, an advanced option has now been added to provide the range of the ligand residue numbers to be considered as a single moiety during the entire protocol.
Glycosyl transferase (GT; EC 2.4.x.x) catalyzes the transfer of a sugar moiety during the formation of a glycosidic bond during the biosynthesis of glycoproteins and glycolipid sugars (Campbell et al. 1997).
In case of anaerobically growing cells, images were taken after a 5 10 min delay shown to be optimal for activation of the GFP moiety during the mounting process.
At the end of the treatments, the cardiomyocytes were labelled with EZ-Link-Sulfo-NHS-LC-Biotin (Pierce) instead of EZ-Link-Sulfo-NHS-SS-Biotin to avoid cleavage of the biotin moiety during the processing of the samples.
The carbamate moiety remains bound to the esteratic site of the enzyme for much longer than is the case for the acetate moiety during the hydrolysis of ACh so that the enzyme is inactivated for some time after the parent molecule has disappeared from the circulation.
