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The sLex moieties were detected by mouse anti-sLex antibody, while CD44v was detected using a panel of monoclonal antibodies against various CD44 isoforms including CD44H, respectively.
To identity modification of rTFF1, the glycoprotein sugar moieties were detected with periodic acid stain method on tricine SDS-polyacrylamide gel.
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With R being an aryl type, as in di-benzoyl and di-naphthoyl peroxides, both R and R–(CO O moieties are detected as polymeric end groups.
The colour change resulting from the dissociation of the magnetic nanobeads moieties was detected by the naked eye and analysed using ImageJ analysis software for the purpose of quantitative measurement.
However, a well-defined peak around 1030 cm-1 with a high intensity at around 1030 cm-1, which is also attributed to guaiacyl moieties, was detected and gradually removed with increasing NaOH concentrations [ 32].
Nevertheless, no lipid moiety was detected in TLC experiments carried out for the detection of total lipids of the antimicrobial peptide.
Further analyses using two different immunoblotting protocols revealed that whereas SNX1 was proteolyzed into a 25- and a 50-kDa fragments, a single 50-kDa cleavage moiety was detected for SNX2 (Figure 1b).
Compound 4j that carrying 4-nitrophenyl piperazine moiety, was detected as the most active agent amongst all compounds with the IC50 value of 0.097 ± 0.003 µM for MAO-B while that of selegiline was 0.040 ± 0.002 µM.
We assessed the glycosylation status of Gc-EGFP by immunoblotting with anti-GFP: the fully glycosylated form of Gc-EGFP migrated at 90 kDa and a hypoglycosylated, faster migrating moiety was detected in fish treated with Tm and in control lysates treated with peptide- N-glycosidase (PNGase) to remove all glycans (supplementary material Fig. S1F).
Moreover, the m/z values at 331.25, 359.28 and 387.32, which corresponded to protonated [M+H]+ molecular ions of a HAA containing C10 C8/C8 C10, C10 C10 and C10 C12/C12 C10 moieties, respectively were detected in the ST5 extract (refer to discussion below and Fig. 5).
Interestingly, both additive-derived compounds and anionic moiety derived ones were detected on the worn surface.
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