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As no significant difference in band intensity was visualized on the reciprocal far-Western (figure 2B lanes 4 and 6) the difference in signal levels between PGK moieties could depend on the relative concentrations of actin and PGK truncations.
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Donaghy et al. observed in 2007 high circulating levels of soluble HLA-G (sHLA-G) in HIV-1-infected individuals, and they hypothesized that these levels could depend on the release of the membrane-bound moiety and thus participate to the pathogenesis of HIV by inducing tolerance [ 49].
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