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After RNAi, cells were infected with A. phagocytophilum at a MOI equivalent to approximately 72 hpi in Figure 1.
After 40 h of incubation, the respective dishes were infected in triplicate with YE-PC8 for an hour at an MOI equivalent of 2.0.
Similar(58)
A549 and DMS 79 cells were infected at varying multiplicities of infection (MOIs: equivalent to plaque forming units/tumor cell).
For incubation experiments, the amount of VLPs per cell was given as MOI-equivalents (MOIeq).
Accordingly, after preincubation of target cells with VLPs, the number of VLPs per cell was given as MOI-equivalents (MOIeq).
In some experiments, at the desired day after isolation, monocytes or monocyte-derived cells were pre-exposed for two hours to VLPs where the amount of VLPs per cell was given as MOI-equivalents (MOIeq).
PMN (1×107/ml) were co- incubated with C. pneumoniae at a ratio of 1∶1 (MOI 1), equivalent amounts of heat-killed C. pneumoniae, or with mock infected HEp-2 cell lysates as negative controls.
Consistent with the reported phenotype of SO2, RvΔphoPR was not impaired in its ability to enter macrophages, with a trend for intracellular numbers greater than H37Rv after challenge at equivalent MOI (Fig. S9).
IDCs and MDCs (0.2 × 10 DCs/well) were challenged with 3 μg p24 equivalent (MOI = 8.5) per 10 cells of F-HIV, C-HIV, or mock treated.
Following incubation with inhibitors DCs (1.5 × 10 DCs/group) were exposed to 75 ng (p24CA equivalent, MOI = 0.15) free HIV-1BaL (F-HIV) or opsonized HIV-1 (C-HIV, IgG-HIV, or C-IgG-HIV) and incubated overnight at 37°C.
Following titration, equivalent MOIs were calculated for the second and third generation systems and used to transduce primary T cells.
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