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Shear modulus of polyacrylamide (PAAM) gels has been measured with the network concentration ranging from 0.02 to 1.0 g/cm3.
The shear modulus of polyacrylamide is independent of strain whereas the modulus of fibrin increases as the applied strain increases (Fig. 1E) [1E].
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The elastic Young's modulus of the polyacrylamide substrates used in this study was 0.6 kPa (0.06% bis-acrylamide, 3% polyacrylamide), 3.5 kPa (0.1% bis-acrylamide, 5% polyacrylamide), and 8 kPa (0.3% bis-acrylamide, 5% polyacrylamide) [61], [62].
Although the Young's modulus of the polyacrylamide gels was not varied in this study, further comparison of 2D versus 3D cell behavior can be found elsewhere [25], [29], [33].
The polymer layer formed by the collagen-1 molecules are too thin (<<0.2 µm) to affect the modulus of the polyacrylamide gel (∼70 µm in thickness) that an attached cell feels.
Rheological measurements indicate that the shear storage modulus of the hydrogels typically increases with the percentage of polyacrylamide for a given polymer volume fraction; however, the simultaneous strengthening and embrittling effects of polyacrylamide as a copolymer are more dramatic for the pNaAc/pAAm hydrogels.
Endothelial cells formed stable networks on relatively soft functionalized polyacrylamide gels (Young's modulus of 140 Pa) in the absence of angiogenic biochemical factors (bFGF or VEGF).
Addition of salt to the cationic polyacrylamide system leads to polyacrylamide bentonite aggregates that result from the interaction of polyacrylamide with the negative face charge of bentonitic clay.
Thus, the polyacrylamide-polypyrrole (PAPPy) microparticles combine the conductivity of polypyrrole and the pore size control of polyacrylamide.
Adsorption of polyacrylamide (PAM) has been extensively studied [10, 14].
KCl also offsets the friction reduction properties of polyacrylamide.
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