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The increases in the moduli were coincided with the increase in calcified area in the bone samples.
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PCM elastic moduli were similar in all three zones.
Second moments of area and section moduli were calculated directly from pixel coordinates without assumptions of cylindrical or elliptical geometry.
The compression moduli and loss moduli were calculated from these measurements (Fig. 10 ).
The storage moduli were calculated from the frequency shifts.
At a cross-linking ratio of 2.20, both the shear storage moduli and the elastic moduli were significantly increased compared to a cross-linking ratio of 1.65.
The moduli were obtained with cyclic indentation at a preset small force on an adherent cell.
Gel moduli were determined within the linear viscoelastic regime for each sample.
The elastic moduli were measured using a rheometer (AR-G2; TA Instruments, New Castle, DE).
Higher shear storage moduli were obtained with hydrogels that swell less.
Polyacrylamide gels of defined elastic moduli were made as described (Yeung et al., 2005).
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