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Most (14 out of 20) of the proteins carrying carbohydrate esterase (CE) modules were classified as multifunctional proteins (Table 3), which will be discussed in the subsequent section.
In addition, recurrent modules were classified by gene-diversity to measure the extent to which multiple genes within each module were up-regulated.
Based on these two diversity measures, modules were classified into six possible idealized patterns to enable further investigation of distinct module classes (see Methods).
All GRNs modules were classified by DAVID functional annotation tool [ 17, 18] to perform GO analysis on the basis of "GOTERM_BP_FAT", whilst pathway enrichment analysis was clustered by ToppFun [ 19].
A total of 58 nodes outside these modules were classified as intermodular hubs (Methods, Additional file 1 Table S8), which link to multiple highly interconnected subnetworks and are putatively important regulators of system behaviour [ 36].
According to the types of nodes around the LWDH genes, the modules were classified into three types: the first one is drugs (drug-module); the second one is TFs (TF-module); the third one is a mix of TFs and miRNAs (mixed-module).
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These five subnetworks can be classified into two larger functional modules in Figure 1.
Standby modules are classified as hot, warm, or cold, depending on the level of service restoration [24].
The CBM modules are classified into three, namely type A, type B, and type C, to define CBMs in terms of their binding specificity.
Fifteen cohesin modules are classified as type II and 34 cohesin modules are classified as type I.
Phages in which a known lysogeny module was annotated were classified as putative temperate phages, phages without a known lysogeny module were classified as putative virulent phages [ 38, 39].
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