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From the networks constructed from these data we identified a list of critical modules of regulated genes forming gene sub-networks that were used to identify regulatory genes involved in primordial follicle development.
We extracted modules of regulated genes from gene sets, and compendia.
However, connected modules of regulated genes are less likely than individual genes to be significant by chance and thus the same significance threshold is not applicable.
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Cluster analyses identified modules of coordinately regulated genes and the different modules appear to accomplish distinct cellular functions during follicle assembly.
We used modules of genes regulated by mTORC1, a molecular complex that contains mTOR [ 51], to check if indeed the CIN-positive tumors also have activation of processes downstream of mTOR.
Each module of coordinately regulated genes was subjected to gene network analysis to determine which genes within a module formed a gene network as shown in Figure 7.
One key assumption of our integrative approach is that TGs sharing expression and functional similarity are likely to be regulated by the same TF s), and TFs sharing expression and functional similarity are likely to form functional modules to regulate the same group of TG(s).
The position and velocity of the modules are regulated by controlling the engagement time of the clutches using the pulse width modulation technique.
The Src kinase module is in charge of regulating the activity of the PTKs Lck and Fyn and ensures the TCR activation threshold.
Thus, complex behaviors such as invasion, which are controlled by several different types of modules, are evidently regulated differently when, for example, they are executed by lymphocytes or metastatic cells as in normal cells the process is terminated after some time, whereas in tumor tissues there is continued execution.
Mainly through genetic screens, the Drosophila community has largely contributed to the molecular dissection of the signaling modules that regulate expression of immune genes following infection.
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