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We confirmed that the prepared antibodies recognize and distinguish tau isoforms containing various modules of exon.
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Ni is the number of probes in probe set i. Chip verification was analyzed first for the overall hybridization quality, then for each module on the chip (3′ expression module, exon module, and SNP module).
These observations concur with the concept of exon shuffling, wherein exons encode structural modules of proteins and the loss and gain of introns and the shuffling of exons via recombination events contribute to the complexity of modern day proteomes.
These observations concur with the concept of exon shuffling, wherein exons encode structural modules of proteins and that throughout the evolution the loss and gain of introns and the shuffling of exons via recombination events contributes towards the complexity of modern day proteomes [ 70, 72].
Unless stated otherwise, we define CIS regions to our module genes as being 200 bp downstream and 1,000 bp upstream of the start of exon 1.
We analyzed the sequencing results using the GenomeStudio RNA Sequencing Module (Illumina, Inc., San Diego, CA) that also allowed us to visualize the aligned reads and analyze results of exon counts, genes and splice junctions found in our data.
Discovery of a duplication of exon 21 of ATP1A2 gene.
fragments per kilobase of exon model per million mapped reads.
We designed mutation screening of exon 5 of CHRNA4, exon 5 of CHRNB2, and exon 6 of CHRNA2 in a group of 56 Chinese sporadic NFLE cases.
Percent of exon inclusion was calculated as [exon inclusion/(exon inclusion+exon exclusion)]×100.
In this paper, we define a splicing module as a set of exons that are regulated by the same splicing factors.
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