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Based on the difference in performance between Groups 1 and 2 (who had the same information in interactive versus PDF format respectively), the critical ingredient behind the success of the modules appears to be their interactivity.
This architecture of modules appears to be shared by a large range of animal species and may even apply to the wormlike creature on the basis of the evolutionary divide between protostomes and deuterostomes, the two largest evolutionary streams of bilateral animals with a centralized nervous system.
Thus, correct positioning of the SH2 and kinase domain modules appears to be critical for efficient activation of cytoplasmic tyrosine kinases (Filippakopoulos et al., 2008).
The common ancestor of these GHKL proteins, MutL and topoisomerase ATPase modules appears to have catalyzed structural reorganization of protein complexes and concomitant DNA-superstructure manipulations along with fused or standalone nuclease domains.
Molecular studies have demonstrated that the acquisition or loss of binding sites or entire regulatory modules appears to drive significant changes in gene expression that initiate critical evolutionary transitions, such as elaboration of novel limb structures (Khila et al., 2009; Pavlopoulos et al., 2009; Tanaka et al., 2011).
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In many of these networks, modules appear to be organized around hubs, as can be seen by comparing a and b of Fig. 1.
MiiA modules appear to have been transferred horizontally between species co-habiting in the same niche to create their own MiiA-containing determinants.
Third, the modules appear to result from the compounding of two types of submodules (1 and 2, shown in Fig. 5).
The density of cells ranged from 1.7×107 to 2.3×108 cells/cm3 as the size of the modules varied: smaller modules appeared to enable higher densities of cells than did larger modules (see Table 1).
RelBE modules appear to be autoregulated in an atypical manner compared to other TA systems; however, the molecular mechanisms and potential interactions between different RelBE modules remain to be elucidated.
However, the sequence motifs within both the RelB/RelE and the RelF/RelG operon modules appeared to have additional complexity, including direct repeat (DR), direct complement (DC), and migrated repeat (MR) sequences.
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