To improve the detection specificity, we implemented an additional constraint related to the conserved head-to-tail connection module organisation observed among most of the phages.
Most prominent and concurrent in this respect, is the enrichment of the single GRE and AUX1/2 motifs as well as their related bi- and tripartite-module organisations, in which they are also commonly associated with MREs.
Its sub-modules contain organisations, people, and systems with a focus on the entities actually involved in a clinical trial.
hapE encodes a single PKS module with the domain organisation KS-AT-KR-ACP-TE, which is identical to the PKS organisation of JamP, which displays the highest amino acid sequence similarity with HapE.
Therefore, hapC, encoding an NRPS module with the domain organisation C-A-KR-PCP is proposed to incorporate 2-oxoisovaleric acid, which is then reduced to 2-hydroxyisovaleric acid, producing C12 and C13 of hapalosin.
hapB, encoding a PKS module with the domain organisation KS-AT-CM-KR-ACP, likely incorporates malonyl-CoA to produce C9-C10 of hapalosin, followed by methylation of C9 to produce C11 via the CM domain, and reduction of the carbonyl group of C8 to a hydroxyl group by the KR domain.
When other modules of identical organisation are analysed in this manner, similar patterns are seen.
For quality of studies (see additional File 1), we adhered to the Effective Organisation of Care Group EPOCC) module[ 15] and defined three risk classes: Class I (low risk of bias), Class II (moderate risk of bias) and Class III (high risk of bias).
Constantly criticizing oneself, especially for the feeling that one is never doing enough, will eventually lead to stress and symptoms of depression [ 23].> Next to information and exercises, participants also receive practical information, tips and references to relevant websites and organisations and the modules contain poems or inspiring texts.
Both organisations offer different training modules of roughly 23 hours given by experts, spread over several weeks, on subjects such as the performance of euthanasia, communication with patient and attending physician, and palliative care.
Each lead received the survey by email, and streamed it to all module leads and examination teams within their organisation who summatively assessed clinical communication.
