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Furthermore, each functional module is flanked by unique cloning sites, ensuring flexibility and individual optimization.
The module is flanked by 17-bp perfect direct repeats and the 3'-end is flanked by an asn tRNA.
The insertion module is flanked by sequences with homology to KanMX4, as well as unique restriction sites.
On its 3′ end, the knock-in module is flanked by the same homologous region that is also present on the 3′ end of the CORE6 cassette to allow for recombination at the target site.
This knock-in module is flanked at the 5′ end by a region of the kanamycin resistance gene to allow for homologous recombination at the 3′ end of the CORE6 cassette.
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NOMAD modules are flanked by sites for the restriction enzyme StyI, which make them compatible with a specifically designed destination vector.
In contrast to MoClo, Biobrick modules are flanked by standard type II restriction enzymes, and assembly of two BioBricks via restriction and ligation results in an idempotent new Biobrick module.
In the Xn, Pl, Pa and Xb genomes, the T6SS and XhlA/B modules were both found in a single RGP but they are not located in the same integration site, as these modules were flanked by different genes of the core genome.
To enable assembly with the Golden Gate technology, each level 0 module type is flanked by specific fusion sites (Fig. 1B).
Albi is flanked by the Mediterranean and Atlantic Coasts.
The panel is flanked by door keepers.
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