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The default run module for microsatellite analysis was used.
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The MICdb admin needs to login (with a valid user id and password) to the admin module for management of microsatellite and genome data in the database.
The microsatellite (excluding monomers) containing unigenes were used in a batch module for designing forward and reverse primers employing the microsatellite primer discovery tool of BatchPrimer3 [ 34].
Expressed sequence tags (ESTs) are a source for microsatellite development.
Sequences containing microsatellites were detected using Tandem Repeats Finder version 4.09 (Benson 1999), and primer pairs were designed for microsatellite loci with suitable flanking regions to amplify using FastPCR software version 6.5.94 (Kalendar et al. 2009).
The assembled contigs contained 92 microsatellite loci, 72 of which were selected for microsatellite development.
Statistics for microsatellite diversity.
Primers for microsatellite loci.
Sampling information for microsatellite genotyping.
MICAS3.0, the web-interface to MICdb3.0, provides three different data access modules—'Browse' (search by alphabetical order of genomes), 'Advanced Search' (search by user criteria) and 'Pair-wise Comparison of Genomes' (compare genomes for microsatellite distribution and densities).
The unigenes were mined for microsatellite motifs.
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