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Subsequently, average phase and modulation lifetimes are calculated.

The average ECFP-p65 control phase and modulation lifetimes were 2.43±0.06 ns and 2.98±0.03 ns in agreement with ECFP lifetimes measured previously [10].

The results of the FLIM analysis of the fusion proteins with SYFP2 as the donor and the red fluorescent proteins as acceptor are shown in table 2. Both phase and modulation lifetime are clearly reduced in the tandem fusion constructs, indicating FRET.

The highest lifetime was observed for cpV9 of which the phase and modulation lifetime is increased by 0.23 ns and 0.28 ns respectively.

The presence of two different lifetime populations is also evident from the 1D histogram (figure 3c) and 2D histogram (figure 3d) in which the modulation lifetime is plotted against the phase lifetime on a pixel-by-pixel basis.

First, to get an indication of trends, the average phase lifetime and average modulation lifetime were determined for each BaF/3 cell along with the average values for a number of cells.

From the phase sequence an intensity (DC) image and the phase and modulation lifetime image are calculated using Matlab macros.

From the phase sequence an intensity (DC) image and the phase and modulation lifetime image are calculated [23] using Matlab macros.

From the phase sequence an intensity (DC) image and the phase and modulation lifetime image was calculated using Matlab macros.

Subsequently, average phase and modulation lifetimes (±standard deviation) are calculated.

Subsequently, average phase and modulation lifetimes (± standard deviation) were calculated.