The alternative operations modulate the orientation of side chains that project from the β-strands and also lead to a shifted or in-register organisation of the β-strands.
Here we combined X-ray crystallography, activity measurements, and native mass spectrometry analysis of the wild-type human heterodimeric catalytic domains of sGC (hereafter termed αβGC) to characterize the structural features that modulate the orientation of the catalytic subunits leading to sGC activity and to propose a novel model for sGC regulation.
In this study, it was suggested that pH can modulate the orientation of water molecules in the pore by titrating external His, which would in turn alter the orientation-dependant electrostatic interactions between water molecules, thus affecting their effective binding energies within the pore.
Our study suggests that strain rate and stretching strain play important roles on modulating the crystallization and orientation of amorphous PLA.
Overall, collective results provide evidence that other sGC domains modulate the relative orientation of the catalytic subunits and control the proper orientation of key residues in the catalytic domain for full enzyme activity.
That the incorporation of cholesterol in a GalCer-NFA monolayer could modulate the average orientation of the sphingolipid is in line with the subsequent studies of GalCer-cholesterol interactions conducted by Smaby et al. [44].
In the ErbB system, we modulated the helix orientation with the V664E mutation and with engineered dimers.
This mechanism is compatible with two recent reports indicating that signaling events downstream Plk1 modulate the correct orientation mitotic spindles (Hanafusa et al., 2015; Yan et al., 2015).
This mechanism is also consistent with two recent reports (now cited in the revised manuscript) indicating that signaling events downstream of Plk1 modulate the correct orientation of mitotic spindles.
