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The modulated proteins were identified by tandem mass spectrometry.
In OA HACs treated with GlcN alone, all of the modulated proteins were increased when compared to the untreated cells (Tab. 1).
In the macrophage model, 78.4 % of the modulated proteins were over-expressed.
Modulated proteins were identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/TOF) mass spectrometry.
Modulated proteins were identified using omics technologies (2-DE proteomics) in cartilage and bone from affected foals compare to OC-free foals.
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To further explore the relationship between genetic design and monoterpene production, the relative abundance of the four modulated proteins was measured for each strain during the active phase of fermentation.
Analysis of modulated proteins was performed with String software (http://www.string-db.org).
Proteomic data agree with this finding since several modulated proteins are associated with bone mineralization and bone remodeling.
The data obtained in the SILAC analysis need to be validated for differences in protein expression profiles before the biological roles of the modulated proteins are extensively studied.
The results obtained by our pharmacoproteomic analysis need to be validated for differences in protein expression profiles before the biological roles of the modulated proteins are extensively studied.
In subchondral bone, differentially modulated proteins are mainly involved in developmental processes, cellular component organization, cell-to-cell signaling, cell adhesion, protein metabolic processes, protein transport and response to stimulus (Additional file 1, bone annotation).
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