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As in mammalian cancer cells [ 89], Microsporidia infected RK-13 cells had a modulated hexokinase expression profile compared to healthy cells, possibly to the benefit of the intracellular parasite.
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AKT regulates the localization of GLUT1 in the plasma membrane and hexokinase expression.
Furthermore, our observation was in agreement with several other studies that have shown correlations of [18F] FDG uptake and hexokinase expression in response to MEK inhibition [17].
However, we also found that expression of hexokinase 2 was only partially dependent on ERK, suggesting that the mechanism may involve changes in both hexokinase expression level and post-translational modifications.
The promoter of hexokinase contains both p53 and HIF response elements and both mutated p53 and activated HIF increase hexokinase expression (Mathupala et al, 1997).
Further, the increase in hexokinase expression also correlated with nearly two fold increase in the total hexokinase activity induced by DNP under these experimental conditions.
Hexokinase expression correlated well with cell size, whereas pyruvate kinase Pkm2 displayed a mixed hepatectomy and cell-size effect.
In contrast, we found no change in hypothalamic glucokinase expression following recurrent hypoglycaemia in rat brains, but rather an increase in hexokinase expression [ 62].
Gari, E., Piedrafita, L., Aldea, M. & Herrero, E. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae.
Gari E, Piedrafita L, Aldea M, Herrero E. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae.
We thus evaluated whether also 4-oxo-4-HPR modulated PLAB expression.
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