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BSA could be used to modulate protein corona as an endogenous ligand in NP design simply by mixing or incubating BSA with NPs before in vivo administration to inhibit or induce cellular uptake in specific cell types.
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Besides differences in the particle properties, the protein corona, which modulates the cellular response to particles, may also give rise to these inconsistent results [ 13].
Lee, Y. K., Choi, E. J., Kim, S. H. & Khang, D. W. Effect of the protein corona on nanoparticles for modulating cytotoxicity and immunotoxicity.
The serum protein-containing biological medium also modulates the spectral changes experienced by cell-exposed NPs through the formation of a protein corona on the surface of NPs that mediates NP interactions with cells: PEGylated AuNPs exposed to cells in serum-free conditions experience greater spectral shifts than in serum-containing environments.
NPs administered into the bloodstream become covered by a plasma proteins layer, called protein corona, that lowers their cytotoxicity and, depending on the type of adsorbed plasma proteins, modulates their immunotoxicity and the targeted delivery into tumor cells [206, 207].
Protein corona formation even stealth active targeting effect of AuNPs.
The protein corona is effectively controlled by the ROS level.
Ritz, S. et al. Protein corona of nanoparticles: distinct proteins regulate the cellular uptake.
The Protein Corona around Nanoparticles Facilitates Stem Cell Labeling for Clinical MR Imaging.
The term protein corona is used to describe the coating of the NM surface with protein.
The protein corona can thus be multi-layered.
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