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The ability of compounds purified from these extracts to significantly modulate insulin mRNA levels was confirmed with real-time PCR.
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Insulin mRNA expression in IPCs increased 16-fold, from day 0 to day 12 (P < 0.05).
Leptin has been shown to be able to modulate insulin secretion.
PTB contributes to stabilization of insulin mRNA [6], [28], [40].
Insulin mRNA expression increases, but only to a point.
Consequently, cellular PTB levels, rather than total insulin mRNA levels, may determine actual insulin translation rates.
To determine whether IRE1α is responsible for this chemically induced ER stress decrease in insulin mRNA expression, we used actinomycin D to attenuate mRNA transcription, then challenged the cells with thapsigargin to induce Insulin 1 and Insulin 2 mRNA degradation.
SCFA may modulate insulin sensitivity by reducing fatty acid flux.
In contrast, miR-30d does not modulate insulin secretion.
Along with FFAs, they modulate insulin sensitivity by altering the phosphorylation state of insulin receptor substrates.
Islet insulin mRNA levels were upregulated in FGF21-KO mice.
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