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In the other half environmental cues could rapidly modulate colony morphology in accordance with the complex etiology and rapid turnover of this phenotypic trait in nature [7], [8].
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To test this hypothesis, we treated pluripotent colonies with pertussis toxin or cholera toxin to irreversibly and specifically modulate Gi or Gs signaling, respectively, and assessed colony morphology and formation by time-lapse and confocal microscopy.
For all other morphotypes, however, the association between colony morphology in vitro and survival in vivo was rather loose indicating that gene inactivation may modulate fitness by various modes, the change of morphotype being the most visible but not necessarily the most relevant feature in vivo.
The colony morphology, colony-forming efficiency and colony size were observed and compared.
The numbers of colonies were counted after identifying the characteristic colony morphology of mutans streptococci.
Interestingly, the plasmid cured strain exhibited small colony morphology and displayed slower growth both in vitro and in vivo.
Hasman, H., Schembri, M.A. & Klemm, P. Antigen 43 and type 1 fimbriae determine colony morphology of Escherichia coli K-12.
Salmonella spp. were identified based on colony morphology on selective media and biochemical properties, according to ISO 6579-1 201739. 6579-1 201739
The colony morphology also remained unchanged during culture.
A B AB Variant 1 0 0 12 8 2 0 0 28 2 3 0 0 8 2 *A, colony morphology of A type homokaryon; B, colony morphology of B type homokaryon (not found); AB, colony morphology of heterokaryon; variant, colony morphology different from A type homokaryon and heterokaryon AB type.
Bacterial colonies were selected on the basis of colony morphology and pigmentation.
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