Eco47III, BspEI and BamHI restriction sites were introduced to allow modular exchange of antibody expression cassettes.
This suggests that WSP chimeric structure is primarily due to recombination events involving foreign DNA rather than a modular exchange of hypervariable regions within a single genome.
Therefore, aside from the likely adaptive advantages presented by a modular exchange of this cassette, these specific breakpoints may have been largely predetermined by the underlying mechanisms of mastrevirus recombination.
These restrictions on modular exchange suggest that there is a selective pressure on maintenance of cognate left and right ICE Hptfs4 ends, for example by an inability of hybrid elements to be excised and/or transferred.
Debridement with exchange of modular components is a treatment option that allows retention of the prosthetic components that are fixed to bone.
The surgical strategy for retention of the implant consists of extensive irrigation and debridement of the infected joint, often accompanied by the exchange of modular implant components and application of local antibiotic carriers, such as beads or collagen fleeces.
In recent years, the simpler procedure, with aggressive surgical debridement and exchange of modular parts of the prosthesis, has become more popular in Norway which is in accordance with experience in the literature (Giulieri et al. 2004, Trebse et al. 2005, Toms et al. 2006, Marculescu et al. 2006).
We systematically generated a panel of 13 different pEPI-1-derived vectors, all of them constructed in a modular way enabling the easy exchange of backbone, promoter, or transgene elements by restriction digest with PciI, NheI, BglII, or MluI.
Early revision due to infection in the case of modular prostheses will therefore involve the exchange of a femoral head, an acetabular liner, or both, and the procedure should therefore be reported to the registry.
The modular design of the carrier facilitates the exchange of different targeting moieties and siRNAs to permit its usage in a variety of tumor types.
We conclude a model in which multi-protein building blocks enable rapid and modular self-assembly of adhesion sites and symmetric exchange of these building blocks preserves their specifications and thus the assembly logic of the system.
