DHEAS levels were determined by an automated electrochemiluminescence immunoassay (Modular Analytics E170) with an analytical sensitivity of 0.003 µmol/L.
Adjustment of a Modular Preanalytics (MPA) and Modular Analytics System SWA (Roche Diagnostics) to our laboratory.
Total cholesterol, high-density lipoproteins, low-density lipoproteins and triacylglycerols (TAG) were measured enzymatically (Modular Analytics, Roche, Basel, Switzerland).
Plasma C-peptide and insulin concentrations were measured using two-sided, electrochemiluminescense immunoassays (Roche/Hitachi modular analytics; Roche Diagnostic GmbH, Mannheim, Germany).
Folate levels were determined by competitive protein-binding assays (Modular Analytics E170, Roche Diagnostics).
Urinary creatinine concentrations were determined by using an automated colorimetric method on a Roche/Hitachi Modular Analytics SWA system (Roche Diagnostics Corp., IN, USA).
Serum cortisol, progesterone, sex hormone-binding globulin (SHBG), and insulin were analyzed by electrochemiluminescence immunoassays, on a Modular Analytics E170 (all from Roche AB, Stockholm, Sweden).
Ferritin was measured using the electrochemiluminescence immunoassay (Modular Analytics E170, Roche Diagnostics, Switzerland. Soluble transferrin receptor (sTfR) levels were measured using ELISA Ramco Laboratoriess, TX, USA).
Serum sodium and creatinine concentrations were determined on Roche Modular Analytics p800.
The samples were analyzed for sodium, creatinine, and urea (Modular Analytics, Roche Diagnostics, Mannheim, Germany).
Plasma cortisol was measured by a commercial immunoassay (Modular Analytics E170, Roche Diagnostics, Mannheim, Germany).
