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We evaluated the effect of modifying recommended extraction protocols to reproducibly produce RNA that may be successfully amplified using QRT-PCR.
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The presence of meningitis should not modify recommended antibiotic regimens because the first goal is to obtain clearance of pathogens from blood cultures.
Several of these departments mention modifying the recommended room equipment to fit their situation, sometimes because of classroom budget/space restrictions or changes in technology (for example, incorporating the use of smart boards and using smart phones for polling).
By modifying the recommended cDNA synthesis protocol, it is possible to label targets in reverse orientation as what would be labeled according to the standard protocol.
By modifying the recommended cDNA synthesis protocol, we demonstrated that it is possible to label targets in reverse orientation as what would be labeled according to the standard protocol (See Additional file 1: Figure S1).
Therefore, the text was not modified as recommended by the referee.
This definition has been modified from recommended definitions by Krantz and Garcia-Moreno [ 17].
We suggest that OIE review this issue and, if warranted, modify the recommended diagnostic methods.
Author's response: The text was modified as recommended and the legend to Figure 2 corrected.
As in our study a modified protocol, recommended by the manufacturer, was used to reduce the risk of PCR inhibition.
The sections were then stained and scored three times in accordance with a modified Osteoarthritis Research Society International OARS I) recommended scoring scheme for cartilage degradation in guinea pigs [ 24].
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