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After 10 min of incubation at room temperature, the suitable concentration of modifying reagents was added and incubated for further 30 min.
Development of low cost, environmental friendly crystal growth modifying reagents that are effective to HAp's microstructure regulation is highly crucial.
The effect is not dependent on the functional groups of the modifying reagents used (none, amino, carboxyl).
Primary aliphatic amines such as hexamethylenediamine and quaternary ammonium salts have been the most effective modifying reagents in the preparation of MMT/HMDA composite.
An alternative way for surface modification can be attained by coating NPs with surface modifying reagents which trigger toxicity to bacteria, and can cause differences in Zn2+ ions release and ROS generation [85].
In the current study, we demonstrated that human somatic cells can be reprogrammed to a stem cell-like state by a combined treatment with epigenetic modifying reagents (DNMT and HDAC inhibitors) and hESC extracts.
Similar(53)
These results are consistent with recent work using the thiol modifying reagent pCMBS which showed that the extent of exposure of S4 in hERG during depolarization is similar to other Kv channels and KvAP [32].
Static cysteine modifications of either carbamidomethylation (IAM-alkylation, +57.0215 Da) or ethanolyl (IE-alkylation, +44.0262 Da) were included on the basis of which modifying reagent was used.
For free glycerol, we modified reagents by Roche (Indianapolis, IN) for glycerol-blanked triglycerides.
In order to optimize the ratio of the modified reagent and the enzyme, effects of the concentration of the modified reagents on activity at a fixed enzyme concentration were investigated.
Substrate protection studies were carried out by incubating the β-xylosidase with excess amount of substrate pNPX for 10 min followed by treatment with corresponding modified reagent.
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