Citrullinated proteins on the membranes were detected by Western blotting by using anti-citrulline (modified) detection kit (Upstate Biotechnology, Lake Placid, NY, USA).
Here we use a modified detection rule for the benchmark algorithm.
In [16] we presented a modified detection strategy for dIDM-STC, adopting the ideas from [13, 14] and incorporating the decoding reliability of the relays into the detection at the destination.
The modified detection rule at R k is given by begin{array}{*{20}l} left[{textbf{x'}},{textbf{y'}}right] = mathop {arg min }limits_{{x_{j},y_{j}} in mathcal{C}} mathop Delta left textbf{X},textbf{Y}right).
Citrullination of the recombinant CapZα-1 was estimated by Western blotting using an anti-citrulline (modified) detection kit (Upstate Biotechnology).
Deiminated proteins were identified with an anti-citrulline (modified) detection kit (catalogue no. 07 -390; Upstate, Lake Placid, NY, USA).
We developed a modified detection method based on polyacrylamide gel staining with ethidium bromide to make this technology more accessible and describe strategies for TILLING in polyploid genomes.
Given that only a small proportion of any given substrate population is usually SUMO modified, detection of conjugated forms of proteins is notoriously difficult without prior enrichment.
Custom DO and commercially available pH sensor spots were used, with a modified detection fiber for OD with a claimed linear range up to 40.
The tissue, either in whole-mount form or in frozen or paraffin sections, is hybridized to an LNA™ modified detection probe, labelled with a digoxygenin (DIG) molecule, and followed by detection with an anti-alkaline phosphatase-conjugated (AP) antibody (Weston et al, 2006).
