Studies on ADAM8 and other metalloproteinases revealed that metalloproteinase activity alone can promote cell attachment, cell outgrowth and cell migration [36], [37], [38], [39], [40] by either direct modification of ECM components such as fibronectin [36], or from proteolytic release of cell adhesion molecules that indirectly modify cell attachment and migration [37], [38], [39], [40].
The in vitro study of hMSCs demonstrated that the RGD modified alginate can improve cell attachment, growth and increase angiogenic growth factor expression.
Demonstrating cell repulsive effect at the mPEGA compositions higher than 7%, the modified surfaces improved MC3T3 cell attachment, proliferation and differentiation, which reached maxima at the mPEGA composition of 5 7%.
Although in vitro data provided support only for transient increased endothelial activity or cell attachment, modified graft surfaces elicited better endothelial and endothelial-like cell attachment in vivo.
Meanwhile, the deposition of this amorphous thin film on a patterned Si surface was found to effectively modify the cellular behaviors, including cell attachment and cell proliferation.
In vitro, the growth factor modified fibrin micro-beads enhanced cell attachment and the migration of human urinary tract smooth muscle cells, however, no change of the cellular metabolic activity was seen.
In-vitro cell culture study was carried out to evaluate biocompatibility and cell attachment onto the modified surface.
Based on oil-in-water emulsion, uniform poly(dimethylsiloxane) (PDMS) beads were prepared using a simple fluidic device and then modified with polydopamine (PDA) to improve cell attachment.
As the adherens junctions are assembled, the cadherin-catenin complex could also actively transform the actin cytoskeleton [ 26], resulting in good protrusion and stretching of hBMSCs and ultimately expanding the cell attachment on the modified polymer surface.
After being modified with dopamine, the protein adsorption amount, cell attachment performance, and mineralization ability of the dopamine-modified highly porous HAP network can be greatly improved.
In a cell transplantation setting, these bio-inert properties can be modified by binding growth factors and incorporating cell attachment and enzyme-sensitive peptide sequences to allow the cells of interest to attach, proliferate, and function [ 63].
