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Finally, by modifying a method described by Blackwell and Horgan [49], we succeeded in expressing PNO of G. geneura without the signal peptide in at least a partially soluble and active form.
Sucrase and Glucoamylase activities were assayed by modifying a method developed by Dahlqvist [ 22].
Therefore, spiked MEM was extracted with a threefold excess either of toluene or n-hexane, modifying a method of Sommerburg et al. [ 31].
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To quantify chemical acetylation kinetics, we modified a method previously developed for determining acetylation stoichiometry.
Here we modified a method from Yu et al. 2007 [ 22].
The 5' and 3' ends of the cDNA of CCT subunits were obtained by a modified RACE method using a First Choice ® RLM RACE KitAmbion).
The dialysis bag technique was compared to a modified cylinder method using a standard dissolution apparatus.
Creatinine measurement was performed using a modified Jaffe method on a Beckman Coulter AU680.
The effective TD is measured using a modified Ångström method, i.e. a modified temperature oscillation method.
The samples were then mineralized in a microwave oven using a modified EPA method 3051.
Protein values were determined by a modified Lowry method [ 32], which provides a linear photometric response.
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