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delete-duplicates is like remove-duplicates, but delete-duplicates may modify sequence.
(remove-duplicates "aBcDAbCd" :test #'char-equal :from-end t) => "aBcD" delete-duplicates might destructively modify sequence.
Protein engineering is an emerging discipline that dovetails modern molecular biology techniques with high-throughput screening, laboratory evolution technologies, and computational approaches to modify sequence, structure, and, in some cases, function and properties of proteins.
To modify sequence length we used PRAAT DSP package v. 5.1.29 (Boersma & Weenink 2011).
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Four modified sequence specific primers (SSP) pairs were designed for the selective amplification of coeliac disease associated alleles (DQA1*05, DQB1*02, DQB1*03 02 alleles), and human growth hormone (positive control).
We prepared two modified DNA sequences in which 5'-CA-3' and 5'-GT-3' were removed from the 6x segment: modified sequence I and modified sequence II (Fig. 1, modified I and II).
Twenty-seven chinsertionnsertion domain sequences, which include four experimentally determined structures and span five kingdoms, were aligned and analyzed using a modified sequence entropy parameter.
Several trials are currently testing the WT1 235–243 natural sequence and modified sequence [17], [18], [19] in patients who are HLA-A2402; another set of studies tested the WT1 126–134 natural sequence [20] and modified peptides in patients who are HLA-A0201 [21]. HLA-A0201 [21]
Target/mutant: sequence of the wild-type gene and the modified sequence or mutant.
Additional file 4 shows the detection rate for all modified sequence motifs, including 6mA.
Porcine trypsin (modified, sequence grade from Promega) was added and incubation continued at 37 °C overnight.
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