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1 A trial of low sodium diet may be worthwhile in elderly subjects with mild (Phase I) ISH: 39 particular indications would include a high sodium excretion rate on usual diet, a willingness to modify salt intake, and a history of gout or diuretic-induced adverse events.
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Purification of DNA was carried out using a modified salt precipitation protocol as described in [52].
DJ-1 deficient and DJ-1 WT MEF were fixed in 2.5% glutaraldehyde in Hank's modified salt solution (HMSS), postfixed in 1% OsO4 in 0.1 M cacodylate buffer, scraped off, centrifuged and dehydrated in a series of ethanol.
DNA was extracted from peripheral blood according to a modified salt precipitation method from 502 Danish medical students of both sexes in their first year at Medical school [52].
Genomic DNA was extracted from peripheral blood using a modified salt extraction method [ 29].
The DNA was extracted following a modified salt precipitation protocol as previously described [ 11].
Genomic DNA was extracted from frozen liver of 95 M. rutilus and 390 M. gapperi following a modified salt extraction method [ 24, 47].
DNA was extracted from the adult tissue and from a small piece of tissue of the developing embryo removed from inside the egg, using a modified salt extraction technique (Aljanabi and Martinez 1997) in 96-well plates (ABgene, Surrey, UK).
We extracted genomic DNA from cryopreserved lymphocytes using a modified salt precipitation-extraction method (Gentra Systems, Minneapolis, MN) or from granulocytes using a silica membrane binding method (Qiagen Inc., Valencia, CA).
We have found that SGI inhibits amplification of medium-size genomic DNA fragments and that this inhibitory effect can be reduced by using a PCR mix, denoted here as mix IV, with modified salt composition [ 5].
Genomic DNA was extracted from blood samples collected from patients with TCC and control subjects using the modified salt precipitation method Puregene kit (Nottingham, UK) in the Regional Genetics Laboratory, Leeds.
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