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These modifications directly modify protein expression.
(C ) A diagram representing temperature conditions used in subsequent experiments to modify protein expression levels.
Enzyme treatment during the preparation of the cell suspensions and sorting procedure might decrease the viability of tumor cells and modify protein expression, thereby affecting the population into which the cells are sorted and the ability of these proteins to play a role in tumor formation following transplantation [ 86].
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This is made possible by the use of siRNAs and their utilization of the RNA interference (RNAi) pathway to degrade specific cellular mRNAs [28], [29], thereby modifying protein expression levels and activities.
As shown in Figure 7, 25 mmol/L glucose induces a PKC α/ βII activating phosphorylation without modifying protein expression.
During these phases of SCI, astrocytes undergo a series of morphological changes and exhibit significantly modified protein expression in a process termed reactive astrogliosis.
These mutations may impair protein function by altering the catalytic activity, the propensity to bind to physiological partners, as well as by affecting steady-state levels (by modifying protein expression or stability).
These results suggest the operation of a physiological mechanism that may adjust enzyme and/or protein expression to modify protein digestibility, and may facilitate design of infant formulae, closer to maternal milk, particularly for premature infants.
On the level of the whole organism, one of the best ways to modify protein activity is to vary the expression pattern.
Thus, cell free expression can be used to modify protein solubility, stability, and pharmacokinetics of therapeutic proteins.
Rather than modify protein structure and function, these noncoding variants frequently impact regulatory elements and thereby alter the quantitative and spatiotemporal regulation of gene expression.
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