To observe growth kinetics within the host, we modified the procedures of Uchiyama et al.[ 49].
In order to make the dual fluorescence assay more convenient, we modified the procedures of plasmid construction so as to speed up the workflow.
We modified the procedure of John et al. (John, J., Sohmen, R., Feuerstein, J., Linke, R., Wittinghofer, A., and Goody, R. (1990) Biochemistry 29, 6058-6065) for the preparation of nucleotide-free ras such that the procedure can now be applied to 1000-fold smaller quantities of protein.
To overcome these drawbacks, Anitua developed plasma rich in growth factors (PRGF) by modifying the procedure of PRP preparation [2].
To this aim, we have slightly modified the procedure of section 4.3 in such a way that, at each iteration, only one keypoint of each match at a time is manipulated.
Native proteins were separated by modifying the procedure of Laemmli (12), where gel electrophoresis was performed under non-reducing and non-denaturing conditions.
Because of length variations between the sequences, we modified the procedure of Ding et al. [ 48] and defined two sequences as paralogs if the aligned regions were over 70% of the shorter sequences.
By modifying the procedure for in utero electroporation of rodents, we recently created a procedure for in utero electroporation to express transgenes in post-mitotic neurons of the ferret cerebral cortex (Kawasaki et al., 2012).
Synthesis began with acetylation of the phenol moieties of fluorescein, modifying the procedure reported by Tour using acetic anhydride and pyridine (>15 g scale, >95% yield), with a mild acid wash being the only work-up necessary (Scheme 1).
Hence, we significantly modified the existing procedures of nanoparticle synthesis in order to obtain palladium nanoparticles of cubic shape (PdC), gold rod-like nanoparticles (AuNR), and nanoparticles with a silver core and a gold shell (AgAu) with a donut-like appearance in TEM (Fig. 1).
Feed-back from the tutor training was used to modify some of the procedures of implementation of the model.
