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Modifier mutants were isolated from an extension of a pilot F1 clonal screen using ethyl methanesulfonate (EMS) as the mutagen (Bülow et al. 2002).
Balanced males from each of the 13 modifier mutants were mated to w; msl1L60/ CyO, w; msl2/ CyO, or w; mle/ CyO virgins, and the presence or absence of flat wing males was scored.
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We tested whether the red eye phenotype seen in our MSL1 modifier mutants was due to enhanced cis spreading from roX1 transgenes, as observed in roX1 roX2 mutants.
This shows that the red eye colour phenotype of modifier MSL1 mutants is not the result of enhanced long distance MSL spreading around the GMroX1 transgene.
Modifier of pma1 (mop) mutants were isolated as enhancers of the mutant phenotypes of pma1 mutants.
The subset of modifiers that produced phenotypes in the absence of the Dys mutants were crossed to meiotic mapping stocks and subsequently mapped using those phenotypes.
The New Mutants are the outcasts.
Another practical application of ENU mutants is for identifying new loci that either suppress or enhance a disease phenotype of interest, also known as a modifier screen.
The jM1 mutant was backcrossed twice into the jba-1D/+ parental background and the modifier was shown to act as a single nuclear trait.
Four of these modifier mutants, Mod59, Mod111, Mod130 and Mod140, had phenotypes in the absence of the dystrophin mutant.
However, chromosome I showed no significant change in volume in these histone modifier mutants.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com