Sentence examples for modified with biotin from inspiring English sources
Probes modified with biotin on the 5′ or 3′ terminus were synthesized and purified via HPLC by the GenScript Company (Nanjing, China).
For the biotin-streptavidin immunoassay, its LSPR extinction spectra are plotted in Fig. 4. The nanohole sample modified with biotin had an LSPR peak at 567 nm.
We designed a pair of primers according to the invA gene of Salmonella bacteria: one was modified with digoxin, and the other was modified with biotin.
The amine end-groups of poly(MMA) chains were also modified with biotin units to ensure selective connection points for streptavidin molecules.
As the initiator strand, reporter probe propagated a chain reaction of hybridization events between the two hairpin probes modified with biotin, and determined whether long nicked DNA polymers were formed.
Using terminal protection of small molecule-linked DNA chimeras, which can protect DNA from degradation by various exonucleases when the small molecule moieties are bound to their protein target, we designed a loop probe, where the 3′-end was modified with biotin to resist digestion by exonucleases in the presence of target SA.
In addition the results agreed very well with those obtained when such duplexes were analysed directly as bimolecular complexes, i.e. when the target, modified with a biotin, was directly immobilized onto a streptavidin sensor chip surface rather than captured by an anchor.
Two photo-reactive peptides, also modified with a biotin handle for pull-down studies, formed covalent interactions with MHC molecules on live cells and provided kinetic data showing the rapid clearance of the peptide-MHC complex.
For immobilizing these aptamers on the sensor surfaces, each sequence was modified with a biotin at the 5′ end and five consequent adenines were used as the spacer for yielding well steric accessibility of the aptamer.
Two detection approaches were evaluated using the dendrimers: (1) dendrimers modified with anti-biotin antibodies for detection of biotinylated bound probes, and (2) dendrimers modified with 45-base long oligonucleotides designed from the single-copy probes, for direct detection of the target region.
Purified XPA was modified with NHS-biotin in the presence and absence of each junction DNA.
