Values of the modified wash-off coefficient are presented for all combinations of rainfall intensities and surface slopes, which can be transferred to other urban catchments with similar conditions.
6 The modified wash test permits evaluation of the whole scalp.
ChIP was performed using the LowCell#ChIP kit (Diagenode) with modified wash conditions (see methods in the supplementary material).
Complexes were then collected by centrifugation and washed firstly in Tris-buffered saline/1% NP40 and then once in modified wash buffer (100 mM Tris, pH 7.4, 50 mM NaCl, 1.5 mM MgCl2, 1 mM ethylenediamine tetraacetic acid, 0.5% NP40).
After pre-spinning homogenized samples at 10,000 rpm at 4°C for 10 minutes to remove fat and cell debris, total RNA was extracted from the supernatant according to the manufacturer's protocol (Invitrogen, Carlsbad, CA, USA), modified by washing the final RNA pellet in 80% ethanol.
In order to distinguish binding from kinetic trapping, we modified the washing volume.
The modified nHA after washing with PBS was lyophilized to obtain white powdery solids of GA-functionalized nHA (nHA-GA) (Fig. 1).
FRC was measured using a ventilator-integrated modified nitrogen wash-in/wash-out method (Engström Carestation™, GE Healthcare, Madison, WI, USA) [8].
We determined the presence of B. procyonis eggs in fecal samples by a modified detergent wash flotation procedure using Sheather's sugar solution, specific gravity 1.25 1.27 (18, 19 ).
Chiumello and coworkers [ 13] compared EELV measured by the conventional helium dilution technique with that obtained by a modified nitrogen wash-out/wash-in system in 30 patients with ALI/ARDS.
