Sentence examples for modified was added from inspiring English sources

When genomic DNA isolated from the A13 cell line that was not SB modified was added, amplification under the same conditions was not observed (Additional file 2: Figure S2).

Sequencing grade modified trypsin was added according to the manufacturer's instructions (Promega), and the samples were digested overnight at 37 °C.

Finally, sequencing grade modified trypsin was added at a protein-to-enzyme ratio of 50 1, followed by incubation overnight at 37 °C.

After 10 min of incubation at room temperature, the suitable concentration of modifying reagents was added and incubated for further 30 min.

The modified tau protein was added to 26 bp DNA for EMSA as mentioned in Figure 1.

Sequencing grade modified trypsin (Promega) was added at an enzyme-to-substrate ratio of 1 25.

After the Matrigel solidified, modified M87 media was added to each well and the cells were cultured overnight.

Ice-cold lysis buffer (modified RIPA buffer) was added to the cells (1 mL per 10 cells/100 mm dish/150 cm flask).

Twenty micrograms of sequencing grade modified trypsin (Promega) was added, pH was adjusted to 8.5 when necessary, and samples were allowed to digest overnight at 37 °C.

This specific rheological behavior is not observed in the case of stable grout or when viscosity modifying agent was added for stabilizing unstable grout.

Subjects were followed for 12 months or until either another disease modifying antirheumatic drug was added or leflunomide was ceased.