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The modified threshold cycle value (Ct) of each sample (Ct of hB2M divided by Ct of GAPDH) was used to calculate the percentage of human cells in murine tissue based on the standard curve.
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Threshold cycle (Ct).
The threshold cycle (Ct) values were determined.
The CFX96 software was used to calculate threshold cycle values.
ΔCt is the threshold cycle of the target gene subtracted from the threshold cycle of the housekeeping gene.
The threshold cycle value (Ct) for each reaction reflects the cycle number in which the fluorescence exceeds the threshold.
The threshold cycle (Ct) is defined as the fractional cycle number at which the fluorescence passes the fixed threshold.
ΔRn threshold was set at 0.02 to obtain the corresponding Ct (threshold cycle) values.
The threshold cycle (Ct) is the cycle at which a significant increase in fluorescence occurs.
The threshold cycle value of the individual measurements did not exceed 0.5 amplification cycles.
Threshold cycle values were averaged.
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