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Since then, he and his colleagues have modified the sequences of influenza viruses to bind to a natural microRNA expressed in humans and mice, in essence developing a virus that's knocked down by the body's natural microRNA.
These results indicate that multiple TEs may have considerably modified the sequences and structures of the ANRIL exons, but their impact on the global structure of ANRIL remains unclear.
Similarly effective were more subtle experiments that modified the sequences of two genes, orc1 and kelch13, which have putative roles in gene silencing and emerging resistance to artemisinin, respectively.
Because an lncRNA could fold into many different structures, making structural prediction difficult, we examined how the insertion of TEs modified the sequences and structures of the ANRIL exons.
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We modified the sequence the amino acid sequence of this peptide (KNYSSSISSIHAC), by replacing and deleting amino acids to obtain higher LPS-binding affinity and greater resistance to protease digestion.
To obtain longer read lengths, we modified the sequencing instrument as detailed in the Supplementary Material.
These transposons not only modified the sequence and structure of ANRIL but also caused the gene to become highly conserved.
We developed this further and modified the sequencing depth estimation by assuming equivalent spike-in-molecules/cell in each experimental set.
In order to define the product of the TES reaction in more detail, we modified the sequence of the branch point to introduce an interrupted site for the restriction enzyme SacI across the junction.
This positional information could be useful in detecting CNEs over large evolutionary distances, under the assumption that evolution sometimes proceeds by modifying the sequences of existing cis-regulatory CNEs without significantly changing their relative position [ 31].
Genome editing with the CRISPR/Cas9 system is making it possible to modify the sequence of genes linked to these disorders.
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