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We modified the sequence the amino acid sequence of this peptide (KNYSSSISSIHAC), by replacing and deleting amino acids to obtain higher LPS-binding affinity and greater resistance to protease digestion.
These transposons not only modified the sequence and structure of ANRIL but also caused the gene to become highly conserved.
In order to define the product of the TES reaction in more detail, we modified the sequence of the branch point to introduce an interrupted site for the restriction enzyme SacI across the junction.
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Since then, he and his colleagues have modified the sequences of influenza viruses to bind to a natural microRNA expressed in humans and mice, in essence developing a virus that's knocked down by the body's natural microRNA.
To obtain longer read lengths, we modified the sequencing instrument as detailed in the Supplementary Material.
We developed this further and modified the sequencing depth estimation by assuming equivalent spike-in-molecules/cell in each experimental set.
Because an lncRNA could fold into many different structures, making structural prediction difficult, we examined how the insertion of TEs modified the sequences and structures of the ANRIL exons.
These results indicate that multiple TEs may have considerably modified the sequences and structures of the ANRIL exons, but their impact on the global structure of ANRIL remains unclear.
Similarly effective were more subtle experiments that modified the sequences of two genes, orc1 and kelch13, which have putative roles in gene silencing and emerging resistance to artemisinin, respectively.
Genome editing with the CRISPR/Cas9 system is making it possible to modify the sequence of genes linked to these disorders.
The program does not modify the sequence of the images, except to invert the sequence if the first energies are in a decreasing order.
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