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We modified the classical calcium phosphate transfection method by detaching adherent chondrocytes with trypsin and resuspending in CaPo4 nucleic acid precipitate followed by readherence for 24 h.
Herein, to meet the need of high concentrations, we modified the classical citrate reduction method and synthesized uniform GNPs from tenfold concentrated precursor (2.5 mM HAuCl4) via adding sodium hydroxide and controlling the temperature.
To investigate the effect of different levels of infection rate and the duration of immunity on the benefit of avoiding exposure to pathogens, we modified the classical susceptible-infected-recovered (SIR) model to integrate different levels of acquired immunity and the boosting effect of attenuated re-infections when it occurs before the level of immunity falls below the protective threshold.
In a previously published pilot study we modified the classical crush technique by developing 'double kissing (DK) double crush' bifurcation stenting [ 10].
Traditionally, CK2 has been described as a constitutively active enzyme composed of two catalytic α and/or α' and two regulatory β subunits [ 6- 8] but mounting evidence has recently modified the classical view of CK2 as a stable tetrameric complex, revealing that the individual CK2 subunits may be asymmetrically distributed and exert independent functions in cells [ 9].
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Intuitively, this was accomplished in Isomap [5] by modifying the classical MDS.
In this paper, the basic concept of DTC will be improved by modifying the classical method.
The fractional order model Equation (1) is in fact obtained by modifying the classical integer order model.
This paper modifies the classical Weibull PDF for wind speeds using a convex combination of Weibull PDFs.
In order to eliminate common operational problems, there are attempts being made to modify the classical tramways.
The output of the controller is the compensator signal S f that modifies the classical voltage reference of the PWM, as shown in Fig. 3.
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