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The fowlpox virus vaccine, DCEP 25 modified strain, was purchased from Merial (Country) and LSDV vaccine, Herbivac® (Ceva), was kindly donated by Deltamune (Pretoria, South Africa).

The modified strain was able to grow in starch as sole carbon source.

Despite the proved expression and secretion of the active α-amylase, the modified strain was unable to grow on starch-based medium with no other carbon source (Figs.  3, 4).

To confirm our assumption that increased intracellular AKG is the direct cause for improved ethylene production, each modified strain was assayed for AKG levels in the absence of EFE.

Recently a modified strain was able to reach a very high carbon to lipid conversion yield (84.7 % of theoretical maximal yield) and very high lipid titers (~55 g/L) under optimized conditions, supporting the feasibility of Y. lipolytica to produce biodiesel [ 24].

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From the data reported, it is deduced that the chief advantage of using the modified strain is that it improves the yield of must/wine extraction, while it also positively affects some variables relating to appearance.

While the lifespan of the genetically modified strains was found to be unaffected under standard growth conditions, increased exogenous hydrogen peroxide stress in the growth medium markedly reduced the lifespan of the PaCatB deletion strain but extended the lifespan of PaCatB over-expressors.

It supervises the development of improved varieties at the Rice Experiment Station in cooperation with the University of California at Davis (no genetically modified strains are among them), and regulates environmental issues, from air and water quality to wildlife habitats.

Consequently the safety of a new enzyme product from these novel genetically modified strains is determined by the background of the production organism.

As described above, a series of modified strains were derived from the robust diploid yeast (Fig. 1b), and they exhibited an alternation from increase to decrease in terms of xylose metabolic capacity.

To prepare vaccine inocula and to compare rates of growth, BCG Tice and modified strains were grown in roller bottles containing Middlebrook 7H9 media with 10% oleic acid-dextrose-catalase (OADC) enrichment supplemented with 0.2% glycerol, and 0.05% Tween80.

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