Exact(3)
To confirm SSL genes identified, we utilized a modified spot assay version of random spore analysis described previously [ 13].
Hits identified in the SGA screens were evaluated by a modified spot assay version of random spore analysis [ 13] using a glc7-E101Q sthatn that co-expressed GLC7-res (YNS100) (Additional File 3) and finally scored as synthetic sick/lethal (SSL) or rejected as false positive.
To examine the effect of ligand position-dependent phosphorylation of the PDZ ligand, Volkmer and coworkers developed a modified SPOT synthesis technique that generated 3 arrays, each containing the 100 PDZ-binding sequences and also all possible phosphorylated variants for the 3 PDZ domains from AF-6, ERBIN, and SNA-1 proteins [ 38].
Similar(57)
The resulting modified spots were inspected ex situ by scanning electron microscopy (SEM).
The laser has high beam uniformity, short wavelength, and the ability to modify the spot size to track an imploding pellet.
Burn severity polygons on this final version (modified based on spot field evaluation of soil condition) exemplifies the greater detail and resolution possible by this improved method.
The artists of that wave, for all their differences, share a peculiar cast of confident but highly deliberate audacity, modifying, with spot-on accuracy, the aesthetic regimes of the New York art world of the time.
Following modifications to the design of the MCF, experiments wereconducted to evaluate the performance of the modified wheel in spot-polishing fused silica glass.
The labeling is continuous across the modified surface with spots of higher fluorescence (Figure 4e,f).
Because each gel has the same spot pattern (modified by the warping transform) a matching of the spots is quite easy and results in 100% spot matching and complete expression profiles.
The basic chip design consisted of 37 collagen spots (300 μm in diameter) in a hexagonal arrangement on a glass substrate; the region without collagen-spots was modified by polyethylene glycol to create the non-adhesive surface.
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