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Having high mass spectral measurement quality has increased the reliability and efficiency of PTM identification at the peptide level and, in addition, has permitted the precise localization of modified sites for some intact protein sequences.
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We explored our data set for lysine acetylation site-specific sequence motifs by analyzing the 12 residues flanking the modified site for overrepresentation of specific amino acids relative to the proteome background distribution.
We found 38 amino acid changes in the axolotl protein that corresponded to mutations present in human cancers or affect protein stability or modify sites of importance for post-translational modifications (table- 2 which also contains scorecons [ 44]).
For real modified sites, we use the Normal distribution to approximate the real distribution of tag numbers (9) To identify genomic sites that are most likely to actually be modified (i.e. real-modified sites), we use sites with large numbers of mapped unique tags.
PHOSIDA is the first software for predicting post-transnationally modified sites.
The amino acids around the modified sites are not obviously conserved, a slight difference between the preferences of amino acids for the ubiquitylation and non-ubiquitylation sites.
Thus, for each modified site under study, the dimension of feature vector representing its sequence context was 21 ∗21.
Efficient access to proteins modified site-specifically with glycans is important in glycobiology and for therapeutic applications.
Fungi facilitate cavity excavation by preparing and modifying excavation sites for cavity excavators.
SS and S1 are initially calculated for rock site and are modified for site class by following formulas: S_{text{MS}} =, F_{text{a}}, S_{S} quad left( { * {text{equation }}16 - 36{text{ IBC}} - 2009} right) (2) S_{text{M1}} =, F_{text{v }} S_{ 1} quad left( { * {text{equation }}16 - 37{text{ IBC}} - 2009} right) (3) 'F a' is site coefficient defined in Table 2.
Thus, Stylonychia H3 variant sequences could predetermine a range of biological functions by restricting the accessibility of modified or unmodified sites for chromatin-modifying proteins to an extent that is to date not known in other eukaryotes.
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