Exact(37)
Immunization with selected modified sequences lowered immunogenicity for particular peptides and revealed residual immunogenicity of incompletely de-immunized modified peptides.
Epitope peptides corresponding to original and modified sequences were validated in HLA binding assays and in immunizations of hemophilic E16 mice, DR3 and DR4 mice and DR3 × E16 mice.
Comparisons of the architecture of the genome, identification of mutated or modified sequences, and pre-and post- transcriptional regulation of gene expression as disease specific biomarkers are revolutionizing our understanding of the causes of disease and are guiding the development of new therapies.
The feasibility of the modified sequences with the proposed acquisition scheme was verified by computer simulations and experiments in different unstable fields generated by the Z0 and Z1 coil current oscillations, which mimic the unstable fields of NMR using externally powered magnets and MRS in the presence of physiological motions, respectively.
Other modified sequences, CA-TG and modified sequences I and II, are also listed under the basic sequence.
In these modified sequences, the red letters in italics represent the four replaced nucleotides in the basic sequence.
Similar(23)
Four modified sequence specific primers (SSP) pairs were designed for the selective amplification of coeliac disease associated alleles (DQA1*05, DQB1*02, DQB1*03 02 alleles), and human growth hormone (positive control).
We prepared two modified DNA sequences in which 5'-CA-3' and 5'-GT-3' were removed from the 6x segment: modified sequence I and modified sequence II (Fig. 1, modified I and II).
Twenty-seven chinsertionnsertion domain sequences, which include four experimentally determined structures and span five kingdoms, were aligned and analyzed using a modified sequence entropy parameter.
Samples were digested overnight with modified sequencing grade trypsin (Promega, Madison, WI), Glu-C (Worthington, Lakewood, NJ), or chymotrypsin (Roche, Switzerland).
Several trials are currently testing the WT1 235–243 natural sequence and modified sequence [17], [18], [19] in patients who are HLA-A2402; another set of studies tested the WT1 126–134 natural sequence [20] and modified peptides in patients who are HLA-A0201 [21]. HLA-A0201 [21]
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