Sentence examples for modified recA strains from inspiring English sources

The most sensitive lux strains found in this study were two modified recA strains, namely DPD2797 and DPD3063 [ 12].

The plasmid pRecA-Htg harbors an E. coli recA wild-type gene encoding an N-terminal histidine-tagged RecA protein (termed here as RecA-Htg variant), whereas the pRecA-GFP plasmid harbors this modified recA gene fused with the gfp gene (green fluorescent protein) that renders a RecA-GFP protein fusion.

Most plasmid libraries are propagated in E. coli recA strains and low expression of a nuclease might be particularly toxic to recA mutants that have no capacity for homology-dependent repair.

UvrA or recA strains of E. coli are more susceptible than the wild-type strain, while the double mutant uvrA recA is the most sensitive strain yet tested.

To rule out the possibility that the defective swarming motility of the recA mutant was associated to defective growth or decreased viability, both the growth rate and viability of the wild-type and Δ recA strains were measured.

This need to P1 transduce Δ hns last also precludes use of recA strains, which typically are used to avoid issues with plasmid recombination.

If the synthetic phenotype observed between sodCI and recA in the animal was due to the inability of the recA strain to induce SOS, then sodCI should also show a synthetic phenotype in the lexA non-inducible strains.

Again, this defect cannot simply be attributed to phagocytic ROS effects; the recA strain was 62-fold decreased in competitive index in vitro (Table 3).

The swarming defect of a Δ recA strain was fully complemented by a plasmid-borne recA gene.

The plasmids were amplified in Escherichia coli EMDH10B-TONA (a recA strain).

For example, the required Cre-expressing recA- strain could have been created by the exchange of the recA+ allele for a recA- allele in the previously constructed Cre-expressing strain MM219Cre [ 3] through generalised transduction by the bacteriophages P1 or T4GT7 from a srl::Tn10 recA strain [ 12].