Sentence examples for modified reagents from inspiring English sources

For free glycerol, we modified reagents by Roche (Indianapolis, IN) for glycerol-blanked triglycerides.

In order to optimize the ratio of the modified reagent and the enzyme, effects of the concentration of the modified reagents on activity at a fixed enzyme concentration were investigated.

Substrate protection studies were carried out by incubating the β-xylosidase with excess amount of substrate pNPX for 10 min followed by treatment with corresponding modified reagent.

Peptides were cleaved from the resin with modified reagent K (TFA 94% (v/v); phenol, 2% (w/v); water, 2% (v/v); TIS, 1% (v/v); EDT, 1% (v/v); 2 hours) and precipitated by adding ice-cold diethyl ether.

After cleaving the peptides from the resin with modified reagent K (TFA 94% (v/v); phenol, 2% (w/v); water, 2% (v/v); TIS, 2% (v/v); 2 hours) they were precipitated with ice-cold diethyl ether and purified to >95% homogeneity by preparative reverse-phase high performance liquid chromatography (RP-HPLC).

After 10 min of incubation at room temperature, the suitable concentration of modifying reagents was added and incubated for further 30 min.

These results are consistent with recent work using the thiol modifying reagent pCMBS which showed that the extent of exposure of S4 in hERG during depolarization is similar to other Kv channels and KvAP [32].

Static cysteine modifications of either carbamidomethylation (IAM-alkylation, +57.0215 Da) or ethanolyl (IE-alkylation, +44.0262 Da) were included on the basis of which modifying reagent was used.

We have synthesised a library of modified Girard reagents incorporating heterocycles larger than pyridine.

The optimal N/P ratio between protonated nitrogen atoms from the dendrimer and phosphate groups from the DNA is known to vary between structurally modified transfection reagents.

Activity of glutamate dehydrogenase (GLDH, Randox; Crumlin, UK) was slightly modified where reagent 1 was premixed with reagent 2, and to this mixture was added to serum and the absorbance monitored for at least 5 min. All animal experiments were approved by the University of Toronto Animal Care Committee.